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anti erk1 2  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti erk1 2
    Anti Erk1 2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+bad/Phospho-Bad+Antibody+Sampler+Kit/pm41898298-93-47-48
    Average 93 stars, based on 15 article reviews
    anti erk1 2 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Fluorescence:

    Article Title: WWOX Induction Promotes Bcl-X L and Mcl-1 Degradation Through a Lysosomal Pathway upon Stress Responses.
    Article Snippet: .. The antibodies used in the detection of specific proteins were anti-Bcl-XL, anti-Mcl-1, anti-Bcl-2, anti-Bax, anti-Bak, anti-Bad (Cell Signaling, Danvers, MA, USA), anti-green fluorescence protein (GFP), anti-WWOX (GeneTex), and anti-β-actin (Sigma). .. The secondary antibodies used were horseradish peroxidase-conjugated goat anti-rabbit IgG or horse anti-mouse IgG (Cell Signaling).

    Incubation:

    Article Title: Genomic copy-number variants drive apoptotic evasion underlying acquired resistance to immune checkpoint inhibitors
    Article Snippet: .. Tissues were incubated with validated primary antibodies, including anti-B2M (Invitrogen, 1:200), anti-GSDMC (Invitrogen, 1:100), anti-FAS (Invitrogen, 1:100), anti-DFFB (Invitrogen, 1:100), anti-CASP9 (Invitrogen, 1:100), and anti-Bad (CST, 1:100), and anti-cleaved CASP3 (CST, 1:500) at 4° C overnight. .. We then incubated tissues with goat anti-mouse IgG (highly cross-absorbed secondary antibody, Alexa Fluor Plus 488, Invitrogen, 1:400) or goat anti-rabbit IgG (highly cross-absorbed secondary antibody, Alexa Fluor 555, Invitrogen, 1:400).

    Article Title: Genomic copy-number variants drive apoptotic evasion underlying acquired resistance to immune checkpoint inhibitors.
    Article Snippet: .. Tissues were incubated with validated primary antibodies, including anti-B2M (Invitrogen, 1:200), anti-GSDMC (Invitrogen, 1:100), anti-FAS (Invitrogen, 1:100), anti-DFFB (Invitrogen, 1:100), anti-CASP9 (Invitrogen, 1:100), and anti-Bad (CST, 1:100), and anti-cleaved CASP3 (CST, 1:500) at 4 ◦ C overnight. .. We then incubated tissues with goat anti-mouse IgG (highly crossabsorbed secondary antibody, Alexa Fluor Plus 488, Invitrogen, 1:400) or goat anti-rabbit IgG (highly cross-absorbed secondary antibody, Alexa Fluor 555, Invitrogen, 1:400).

    Article Title: Deciphering the Mechanisms Underlying the Antitumor Effects of Eucalyptus Essential Oil and Its Component 3-Cyclohexene-1-Methanol Against Human Colon Cancer Cells
    Article Snippet: .. Thereafter, membranes were incubated overnight with indicated primary antibodies (anti-p53, anti-pp53 (Ser15), anti-p27, anti-p21, anti-Cyclin D1, anti-CDK2, anti-CDK4, anti-phospho CDC2 (Tyr15), anti-phospho Rb, anti-TRADD, anti-Caspase 8, anti-Caspase 7, anti-Caspase 9, anti-AIF, anti-Caspase 3, anti-Bad, anti-phospho Bad (Ser112), anti-Cytochrome C, anti-Bik, anti-Bax, anti-Puma, anti-AKT, anti-phospho AKT (Ser473), anti-phospho ERK1/2 (Thr202/Tyr204), anti-ERK1/2, anti-phospho p38 (Thr180/Tyr182), anti-p38, anti-phospho SAPK/JNK (Thr183/Tyr185), anti-HSP90, anti-β-actin) from cell Signaling Technology (Danvers, MA, USA) at a dilution of 1:1000. β-actin and HSP90 were used as the loading control. .. Immunoreactivity was detected by using horseradish peroxidase-conjugated anti-rabbit or anti-mouse IgG (Promega, Madison, WI, USA) and visualized in an X-ray film or using a ChemiDoc MP Imaging System (Bio-Rad, Hercules, CA, USA) after incubation with enhanced Chemiluminescent (ECL, Pierce, Rockford, IL, USA).

    Western Blot:

    Article Title: BH3 mimetics targeting BCL-XL have efficacy in solid tumors with RB1 loss and replication stress
    Article Snippet: Cells were lysed in RIPA buffer (#PI89900, Fisher Scientific) supplemented with protease inhibitor (#PI78437, Fisher Scientific) and phosphatase inhibitor cocktails (#PI78426, Fisher Scientific). .. The following primary antibodies were used for immunoblotting at 1:1000 dilution unless otherwise indicated: anti-RPA32/RPA2 (phospho S33, Abcam #ab211877, 1:2000), anti-H2A.X phospho Ser139 (clone 20E3, Cell Signaling Technology #9718), anti-p53 (Cell Signaling Technology #9282), anti-p21 (clone EPR3993, Abcam #ab109199), anti-Survivin (Cell Signaling Technology #2808), anti-RB1 (clone 4H1, Cell Signaling Technology #9309, 1:2000), anti-Bad (#9239, Cell Signaling Technology, 1:500), anti-BAK (#12105, Cell Signaling Technology), anti-BAX (#5023, Cell Signaling Technology), anti-β-actin (#ab6276, Abcam), anti-BCL2 (#4223, Cell Signaling Technology, 1:500), anti-BCL-XL (#2764, Cell Signaling Technology), anti-BIM (#2933, Cell Signaling Technology), anti-cleaved caspase 3 (#9664, Cell Signaling Technology1:250), anti-MARCH5 (#06-1036, EMD Millipore, 1:500), anti-MCL1 (#5453, Cell Signaling Technology), anti-NOXA (#ab13654, Abcam, 1:250), anti-PARP (#9532, Cell Signaling Technology), anti-phospho-eIF2α Ser51 (#9721, Cell Signaling Technology), anti-PUMA (#12450, Cell Signaling Technology, 1:500), or anti-vinculin (#sc-73614, Santa Cruz Biotechnology, 1:20,000). .. The secondary antibodies were 1:5000 of anti-rabbit (#W401B) or anti-mouse (#W402B) secondary (Promega).

    Control:

    Article Title: Deciphering the Mechanisms Underlying the Antitumor Effects of Eucalyptus Essential Oil and Its Component 3-Cyclohexene-1-Methanol Against Human Colon Cancer Cells
    Article Snippet: .. Thereafter, membranes were incubated overnight with indicated primary antibodies (anti-p53, anti-pp53 (Ser15), anti-p27, anti-p21, anti-Cyclin D1, anti-CDK2, anti-CDK4, anti-phospho CDC2 (Tyr15), anti-phospho Rb, anti-TRADD, anti-Caspase 8, anti-Caspase 7, anti-Caspase 9, anti-AIF, anti-Caspase 3, anti-Bad, anti-phospho Bad (Ser112), anti-Cytochrome C, anti-Bik, anti-Bax, anti-Puma, anti-AKT, anti-phospho AKT (Ser473), anti-phospho ERK1/2 (Thr202/Tyr204), anti-ERK1/2, anti-phospho p38 (Thr180/Tyr182), anti-p38, anti-phospho SAPK/JNK (Thr183/Tyr185), anti-HSP90, anti-β-actin) from cell Signaling Technology (Danvers, MA, USA) at a dilution of 1:1000. β-actin and HSP90 were used as the loading control. .. Immunoreactivity was detected by using horseradish peroxidase-conjugated anti-rabbit or anti-mouse IgG (Promega, Madison, WI, USA) and visualized in an X-ray film or using a ChemiDoc MP Imaging System (Bio-Rad, Hercules, CA, USA) after incubation with enhanced Chemiluminescent (ECL, Pierce, Rockford, IL, USA).



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